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rat anti mouse cd4 rpe  (Bio-Rad)


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    Structured Review

    Bio-Rad rat anti mouse cd4 rpe
    The immunofluorescence staining of CT26 tumors harvested from control and Amuc_C-treated groups. (A) T cell panel. CD3, CD8, and <t>CD4</t> were stained in green, red, and pink; (B) M1 macrophage panel. CD86 and iNOS were stained pink and red; (C) M2 macrophage panel. CD206 and arginase-1 (Arg-1) were stained in red and pink. Cell nuclei were stained with DAPI. (scale bar = 20 μm). Images were quantified randomly in five fields at 20x magnification. All data are presented as mean ± SEM. Statistical significance was calculated by one-way ANOVA with the Tukey test for the comparisons between all groups (ns, not significant; *, p < 0.05; ****, p < 0.0001).
    Rat Anti Mouse Cd4 Rpe, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti mouse cd4 rpe/product/Bio-Rad
    Average 95 stars, based on 398 article reviews
    rat anti mouse cd4 rpe - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "Novel TLR2 agonist Amuc_C derived from Akkermansia muciniphila exhibits potent anti-tumor activity in colorectal cancers"

    Article Title: Novel TLR2 agonist Amuc_C derived from Akkermansia muciniphila exhibits potent anti-tumor activity in colorectal cancers

    Journal: Animal Cells and Systems

    doi: 10.1080/19768354.2025.2578019

    The immunofluorescence staining of CT26 tumors harvested from control and Amuc_C-treated groups. (A) T cell panel. CD3, CD8, and CD4 were stained in green, red, and pink; (B) M1 macrophage panel. CD86 and iNOS were stained pink and red; (C) M2 macrophage panel. CD206 and arginase-1 (Arg-1) were stained in red and pink. Cell nuclei were stained with DAPI. (scale bar = 20 μm). Images were quantified randomly in five fields at 20x magnification. All data are presented as mean ± SEM. Statistical significance was calculated by one-way ANOVA with the Tukey test for the comparisons between all groups (ns, not significant; *, p < 0.05; ****, p < 0.0001).
    Figure Legend Snippet: The immunofluorescence staining of CT26 tumors harvested from control and Amuc_C-treated groups. (A) T cell panel. CD3, CD8, and CD4 were stained in green, red, and pink; (B) M1 macrophage panel. CD86 and iNOS were stained pink and red; (C) M2 macrophage panel. CD206 and arginase-1 (Arg-1) were stained in red and pink. Cell nuclei were stained with DAPI. (scale bar = 20 μm). Images were quantified randomly in five fields at 20x magnification. All data are presented as mean ± SEM. Statistical significance was calculated by one-way ANOVA with the Tukey test for the comparisons between all groups (ns, not significant; *, p < 0.05; ****, p < 0.0001).

    Techniques Used: Immunofluorescence, Staining, Control



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    The immunofluorescence staining of CT26 tumors harvested from control and Amuc_C-treated groups. (A) T cell panel. CD3, CD8, and <t>CD4</t> were stained in green, red, and pink; (B) M1 macrophage panel. CD86 and iNOS were stained pink and red; (C) M2 macrophage panel. CD206 and arginase-1 (Arg-1) were stained in red and pink. Cell nuclei were stained with DAPI. (scale bar = 20 μm). Images were quantified randomly in five fields at 20x magnification. All data are presented as mean ± SEM. Statistical significance was calculated by one-way ANOVA with the Tukey test for the comparisons between all groups (ns, not significant; *, p < 0.05; ****, p < 0.0001).
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    The immunofluorescence staining of CT26 tumors harvested from control and Amuc_C-treated groups. (A) T cell panel. CD3, CD8, and <t>CD4</t> were stained in green, red, and pink; (B) M1 macrophage panel. CD86 and iNOS were stained pink and red; (C) M2 macrophage panel. CD206 and arginase-1 (Arg-1) were stained in red and pink. Cell nuclei were stained with DAPI. (scale bar = 20 μm). Images were quantified randomly in five fields at 20x magnification. All data are presented as mean ± SEM. Statistical significance was calculated by one-way ANOVA with the Tukey test for the comparisons between all groups (ns, not significant; *, p < 0.05; ****, p < 0.0001).
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    The immunofluorescence staining of CT26 tumors harvested from control and Amuc_C-treated groups. (A) T cell panel. CD3, CD8, and <t>CD4</t> were stained in green, red, and pink; (B) M1 macrophage panel. CD86 and iNOS were stained pink and red; (C) M2 macrophage panel. CD206 and arginase-1 (Arg-1) were stained in red and pink. Cell nuclei were stained with DAPI. (scale bar = 20 μm). Images were quantified randomly in five fields at 20x magnification. All data are presented as mean ± SEM. Statistical significance was calculated by one-way ANOVA with the Tukey test for the comparisons between all groups (ns, not significant; *, p < 0.05; ****, p < 0.0001).
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    The immunofluorescence staining of CT26 tumors harvested from control and Amuc_C-treated groups. (A) T cell panel. CD3, CD8, and <t>CD4</t> were stained in green, red, and pink; (B) M1 macrophage panel. CD86 and iNOS were stained pink and red; (C) M2 macrophage panel. CD206 and arginase-1 (Arg-1) were stained in red and pink. Cell nuclei were stained with DAPI. (scale bar = 20 μm). Images were quantified randomly in five fields at 20x magnification. All data are presented as mean ± SEM. Statistical significance was calculated by one-way ANOVA with the Tukey test for the comparisons between all groups (ns, not significant; *, p < 0.05; ****, p < 0.0001).
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    Percentage of CD3 + , CD3 + <t>CD4</t> + , and CD3 + CD8 + T lymphocytes in mice peripheral blood at day 30. ( A ) The representative flow cytometry plots showing CD3 + , CD3 + CD4 + and CD3 + CD8 + T lymphocytes in the peripheral blood. ( B ) Frequencies of CD3 + T lymphocytes. ( C ) Frequencies of CD3 + CD4 + T lymphocytes. ( D ) Frequencies of CD3 + CD8 + T lymphocytes. Data are presented as mean ± SD. Analyses were performed by t tests (NS = p > 0.05; * p < 0.05; ** p < 0.01).
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    Bio-Rad rat antimouse cd4 fitc cd8 rpe
    Percentage of CD3 + , CD3 + <t>CD4</t> + , and CD3 + CD8 + T lymphocytes in mice peripheral blood at day 30. ( A ) The representative flow cytometry plots showing CD3 + , CD3 + CD4 + and CD3 + CD8 + T lymphocytes in the peripheral blood. ( B ) Frequencies of CD3 + T lymphocytes. ( C ) Frequencies of CD3 + CD4 + T lymphocytes. ( D ) Frequencies of CD3 + CD8 + T lymphocytes. Data are presented as mean ± SD. Analyses were performed by t tests (NS = p > 0.05; * p < 0.05; ** p < 0.01).
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    Image Search Results


    The immunofluorescence staining of CT26 tumors harvested from control and Amuc_C-treated groups. (A) T cell panel. CD3, CD8, and CD4 were stained in green, red, and pink; (B) M1 macrophage panel. CD86 and iNOS were stained pink and red; (C) M2 macrophage panel. CD206 and arginase-1 (Arg-1) were stained in red and pink. Cell nuclei were stained with DAPI. (scale bar = 20 μm). Images were quantified randomly in five fields at 20x magnification. All data are presented as mean ± SEM. Statistical significance was calculated by one-way ANOVA with the Tukey test for the comparisons between all groups (ns, not significant; *, p < 0.05; ****, p < 0.0001).

    Journal: Animal Cells and Systems

    Article Title: Novel TLR2 agonist Amuc_C derived from Akkermansia muciniphila exhibits potent anti-tumor activity in colorectal cancers

    doi: 10.1080/19768354.2025.2578019

    Figure Lengend Snippet: The immunofluorescence staining of CT26 tumors harvested from control and Amuc_C-treated groups. (A) T cell panel. CD3, CD8, and CD4 were stained in green, red, and pink; (B) M1 macrophage panel. CD86 and iNOS were stained pink and red; (C) M2 macrophage panel. CD206 and arginase-1 (Arg-1) were stained in red and pink. Cell nuclei were stained with DAPI. (scale bar = 20 μm). Images were quantified randomly in five fields at 20x magnification. All data are presented as mean ± SEM. Statistical significance was calculated by one-way ANOVA with the Tukey test for the comparisons between all groups (ns, not significant; *, p < 0.05; ****, p < 0.0001).

    Article Snippet: The following monoclonal antibodies were used to assess the phenotypes of T lymphocytes, macrophages, and dendritic cells: PE/Cyanine7 anti-mouse CD45 (BioLegend, clone 30-F11, Cat. No. 103114), FITC anti-mouse Ly-6C (BioLegend, clone HK1.4, Cat. No. 128006), rat anti-Mouse CD3: FITC (Bio–Rad, Hercules, CA, USA, clone KT3, Cat. No. MCA500F), rat anti-Mouse CD4: RPE (Bio–Rad, clone RM4-5, Cat. No. MCA2691PE), rat anti-Mouse CD8: Alexa Fluor® 647 (Bio–-Rad, clone YTS169.4, Cat. No. MCA1768A647), PE anti-mouse F4/80 (BioLegend, clone BM8, Cat. No. 123110), APC/Cyanine7 anti-mouse/human CD11b (BioLegend, clone M1/70, Cat. No. 101226), PerCP/Cyanine5.5 anti-mouse I-A/I-E (BioLegend, clone M5/114.15.2, Cat. No. 107626), APC anti-mouse CD11c (BioLegend, clone N418, Cat. No. 117310), and PE anti-mouse CD103 (BioLegend, clone 2E7, Cat. No. 121406).

    Techniques: Immunofluorescence, Staining, Control

    Percentage of CD3 + , CD3 + CD4 + , and CD3 + CD8 + T lymphocytes in mice peripheral blood at day 30. ( A ) The representative flow cytometry plots showing CD3 + , CD3 + CD4 + and CD3 + CD8 + T lymphocytes in the peripheral blood. ( B ) Frequencies of CD3 + T lymphocytes. ( C ) Frequencies of CD3 + CD4 + T lymphocytes. ( D ) Frequencies of CD3 + CD8 + T lymphocytes. Data are presented as mean ± SD. Analyses were performed by t tests (NS = p > 0.05; * p < 0.05; ** p < 0.01).

    Journal: Viruses

    Article Title: Immune Responses to Orally Administered Recombinant Lactococcus lactis Expressing Multi-Epitope Proteins Targeting M Cells of Foot-and-Mouth Disease Virus

    doi: 10.3390/v13102036

    Figure Lengend Snippet: Percentage of CD3 + , CD3 + CD4 + , and CD3 + CD8 + T lymphocytes in mice peripheral blood at day 30. ( A ) The representative flow cytometry plots showing CD3 + , CD3 + CD4 + and CD3 + CD8 + T lymphocytes in the peripheral blood. ( B ) Frequencies of CD3 + T lymphocytes. ( C ) Frequencies of CD3 + CD4 + T lymphocytes. ( D ) Frequencies of CD3 + CD8 + T lymphocytes. Data are presented as mean ± SD. Analyses were performed by t tests (NS = p > 0.05; * p < 0.05; ** p < 0.01).

    Article Snippet: Guinea pig anticoagulated blood samples were obtained on day 30 after initial immunization and stained for 30 min using APC-conjugated rat anti-mouse CD3 antibody, RPE-conjugated rat anti-mouse CD4 antibody and FITC-conjugated rat anti-mouse CD8 antibody (AbD Serotec, Oxford, UK) at room temperature (RT).

    Techniques: Flow Cytometry